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axioimager m2 fluorescence microscope  (Carl Zeiss)


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    Carl Zeiss axioimager m2 fluorescence microscope
    Axioimager M2 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioimager+m2+fluorescence+microscope/software+zen/bio_rxiv__2025__07__11__663895-203-8-7
    Average 90 stars, based on 1 article reviews
    axioimager m2 fluorescence microscope - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: .. Images were acquired with an AxioImager M2 (Zeiss) fluorescence microscope with a 20x/0,8 objective and contrast was adjusted with Fiji software. ..

    Article Title: Transient receptor potential vanilloid 2 (TRPV2) channels modulate the nigrostriatal dopaminergic activity in rats.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Mediator-29 limits Caenorhabditis elegans fecundity.
    Article Snippet: *Corresponding authors: Wei Cao, Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia.. Email: wei.cao@monash.edu; Roger Pocock, Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia.. Email: roger.pocock@monash.edu These authors contributed equally to this work.

    Article Title: Small extracellular vesicles from the human endothelial cell line EA.hy 926 exert a self-cell activation and modulate DENV-2 genome replication and infection in naïve endothelial cells.
    Article Snippet: .. Finally, morphological changes of the cells were evaluated using the immunofluorescence technique with the markers phalloidin (CS 8940S) for actin structure evaluation and ICAM (SC 1511) for adhesion molecule visualization under the Axioimager M2 fluorescence microscope and the software Zen 2012 (Zeiss). ..

    Article Title: Poly-basic peptides and polymers as new drug candidates against Plasmodium falciparum.
    Article Snippet: For nuclear staining, 1:1000 dilution of 1 mg/ ml DAPI (Roche Diagnostics) was included during Poly-L-Lysine labelling. .. Sporozoites were then viewed with a Zeiss AxioImager M2 fluorescence microscope equipped with an oil-immersion Zeiss plan Apo 100 × / NA 1.4 objective or a Zeiss EC plan Neo 40 × /NA 0.75 objective and a Hamamatsu ORCA-R2 camera. .. Optical z-sections with 0.2 μm spacing were acquired using Volocity software (Quorum Technologies, Puslinch, ON, Canada).

    Article Title: Physiologically-Modeled Dynamic Stimulation and Growth Factors Induce Differentiation of Mesenchymal Stem Cells to a Vascular Endothelial Cell Phenotype.
    Article Snippet: Objective: Mesenchymal stem cells (MSCs) represent an attractive option as an endothelial cell (EC) source for regenerative medicine therapies.. However, the differentiation of MSCs toward an ECs phenotype can be regulated by a complex and dynamic microenvironment, including specific growth factors as well as local mechanical cues.. The objective of this work was to evaluate whether Physiologicallymodeled dynamic stimulation (PMDS) characterized by continuous variability in pulse frequencies mimicking the dynamic temporal range of cardiac function would enhance MSC differentiation toward ECs compared to a constant frequency stimulation.

    Article Title: Mediator-29 Limits Caenorhabditis elegans Fecundity
    Article Snippet: .. After DAPI staining, z-stack images of spermatheca were obtained using the AxioImager M2 (Zeiss) fluorescence microscope (40x objective). .. The number of sperm was manually counted using the FIJI (Fiji Is Just) ImageJ 2.14.0/1.54f software.

    Article Title: TPR is required for cytoplasmic chromatin fragment formation during senescence
    Article Snippet: .. For the CM experiment (Figure 2) images were acquired using a Photometrics Prime BSI CMOS camera (Teledyne Photometrics) fitted to a Zeiss AxioImager M2 fluorescence microscope with Plan- Apochromat objectives, a Zeiss Colibri 7 LED light source, together with Zeiss filter sets 90 HE, 92 HE, 96 HE, 38 HE, and 43 HE (Carl Zeiss UK). .. Image capture was performed in Zeiss Zen 3.5 software.

    Microscopy:

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: .. Images were acquired with an AxioImager M2 (Zeiss) fluorescence microscope with a 20x/0,8 objective and contrast was adjusted with Fiji software. ..

    Article Title: Transient receptor potential vanilloid 2 (TRPV2) channels modulate the nigrostriatal dopaminergic activity in rats.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Mediator-29 limits Caenorhabditis elegans fecundity.
    Article Snippet: *Corresponding authors: Wei Cao, Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia.. Email: wei.cao@monash.edu; Roger Pocock, Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia.. Email: roger.pocock@monash.edu These authors contributed equally to this work.

    Article Title: Small extracellular vesicles from the human endothelial cell line EA.hy 926 exert a self-cell activation and modulate DENV-2 genome replication and infection in naïve endothelial cells.
    Article Snippet: .. Finally, morphological changes of the cells were evaluated using the immunofluorescence technique with the markers phalloidin (CS 8940S) for actin structure evaluation and ICAM (SC 1511) for adhesion molecule visualization under the Axioimager M2 fluorescence microscope and the software Zen 2012 (Zeiss). ..

    Article Title: Poly-basic peptides and polymers as new drug candidates against Plasmodium falciparum.
    Article Snippet: For nuclear staining, 1:1000 dilution of 1 mg/ ml DAPI (Roche Diagnostics) was included during Poly-L-Lysine labelling. .. Sporozoites were then viewed with a Zeiss AxioImager M2 fluorescence microscope equipped with an oil-immersion Zeiss plan Apo 100 × / NA 1.4 objective or a Zeiss EC plan Neo 40 × /NA 0.75 objective and a Hamamatsu ORCA-R2 camera. .. Optical z-sections with 0.2 μm spacing were acquired using Volocity software (Quorum Technologies, Puslinch, ON, Canada).

    Article Title: Physiologically-Modeled Dynamic Stimulation and Growth Factors Induce Differentiation of Mesenchymal Stem Cells to a Vascular Endothelial Cell Phenotype.
    Article Snippet: Objective: Mesenchymal stem cells (MSCs) represent an attractive option as an endothelial cell (EC) source for regenerative medicine therapies.. However, the differentiation of MSCs toward an ECs phenotype can be regulated by a complex and dynamic microenvironment, including specific growth factors as well as local mechanical cues.. The objective of this work was to evaluate whether Physiologicallymodeled dynamic stimulation (PMDS) characterized by continuous variability in pulse frequencies mimicking the dynamic temporal range of cardiac function would enhance MSC differentiation toward ECs compared to a constant frequency stimulation.

    Article Title: Mediator-29 Limits Caenorhabditis elegans Fecundity
    Article Snippet: .. After DAPI staining, z-stack images of spermatheca were obtained using the AxioImager M2 (Zeiss) fluorescence microscope (40x objective). .. The number of sperm was manually counted using the FIJI (Fiji Is Just) ImageJ 2.14.0/1.54f software.

    Article Title: TPR is required for cytoplasmic chromatin fragment formation during senescence
    Article Snippet: .. For the CM experiment (Figure 2) images were acquired using a Photometrics Prime BSI CMOS camera (Teledyne Photometrics) fitted to a Zeiss AxioImager M2 fluorescence microscope with Plan- Apochromat objectives, a Zeiss Colibri 7 LED light source, together with Zeiss filter sets 90 HE, 92 HE, 96 HE, 38 HE, and 43 HE (Carl Zeiss UK). .. Image capture was performed in Zeiss Zen 3.5 software.

    Software:

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: .. Images were acquired with an AxioImager M2 (Zeiss) fluorescence microscope with a 20x/0,8 objective and contrast was adjusted with Fiji software. ..

    Article Title: Small extracellular vesicles from the human endothelial cell line EA.hy 926 exert a self-cell activation and modulate DENV-2 genome replication and infection in naïve endothelial cells.
    Article Snippet: .. Finally, morphological changes of the cells were evaluated using the immunofluorescence technique with the markers phalloidin (CS 8940S) for actin structure evaluation and ICAM (SC 1511) for adhesion molecule visualization under the Axioimager M2 fluorescence microscope and the software Zen 2012 (Zeiss). ..

    Staining:

    Article Title: Mediator-29 limits Caenorhabditis elegans fecundity.
    Article Snippet: *Corresponding authors: Wei Cao, Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia.. Email: wei.cao@monash.edu; Roger Pocock, Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia.. Email: roger.pocock@monash.edu These authors contributed equally to this work.

    Article Title: Mediator-29 Limits Caenorhabditis elegans Fecundity
    Article Snippet: .. After DAPI staining, z-stack images of spermatheca were obtained using the AxioImager M2 (Zeiss) fluorescence microscope (40x objective). .. The number of sperm was manually counted using the FIJI (Fiji Is Just) ImageJ 2.14.0/1.54f software.

    Immunofluorescence:

    Article Title: Small extracellular vesicles from the human endothelial cell line EA.hy 926 exert a self-cell activation and modulate DENV-2 genome replication and infection in naïve endothelial cells.
    Article Snippet: .. Finally, morphological changes of the cells were evaluated using the immunofluorescence technique with the markers phalloidin (CS 8940S) for actin structure evaluation and ICAM (SC 1511) for adhesion molecule visualization under the Axioimager M2 fluorescence microscope and the software Zen 2012 (Zeiss). ..



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    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
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    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
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    Image Search Results


    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under fluorescence microscope show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.

    Journal: Clinical Ophthalmology (Auckland, N.Z.)

    Article Title: TRAP1 Improves Diabetic Retinopathy by Preserving Mitochondrial Function

    doi: 10.2147/OPTH.S521660

    Figure Lengend Snippet: HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under fluorescence microscope show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.

    Article Snippet: Fluorescence images were captured using a Zeiss AxioImager M2 fluorescence microscope (Zeiss, Germany) with the following parameters: Oxidized state (green fluorescence): Excitation wavelength of 488 nm, emission filter of 500–550 nm;- Reduced state (red fluorescence): Excitation wavelength of 581 nm, emission filter of 590–650 nm.Images were analyzed using Zen 3.1 software and quantified with ImageJ software.

    Techniques: Cell Culture, Staining, Fluorescence, Microscopy, Polymer, Control, Western Blot, Extraction, Quantitative RT-PCR, Expressing